There are a lot of great discussions about the ever-increasing amount of LGBT characters in anime, the roles they play, and what they add to the shows they’re in. I’m not doing any of that right now! Below is a list of every anime that has LGBT+ characters/relationships for your reference, somewhat grouped by general categories. Will be edited/reposted as needed.
These shows include a gay or lesbian romance! (in no particular order)
Sakura Trick – It’s a romance story between two girls who keep making out.
Yuri!!! on Ice – Competitive figure skating sports show with a gay love romance secondary plot.
Love Stage – a case of mistaken gender sparks a question of the main character’s sexuality in this romantic comedy.
Sasameki Koto – A lesbian romance story, but also a comedy about several lesbian high schoolers making a club for lesbians to bond and oogle girls and be gay in. Double gay as most of the main cast is gay.
Scum’s Wish – The main character has a relationship turned romantic/sexual with her lesbian friend who is pursuing her, though through this she discovers she is not gay. They value their relationship though it then gets awkward, but is a very realistic portrayal.
Sukisho (Suki na Mono wa Suki Dakara Shou ga Nai!!) – a romance mainly but with drama and comedy elements as well. It’s about two boys who fall for each other through memory problems, moves, and multiple personalities.
Junjou Romantica – Two adult men end up living together and fall into a (gay) relationship.
Sekaiichi Hatsukoi – Onodera starts a new job and it’s not what he expected, especially when he realizes his new boss is his first love. They have gay romance
Netsuzou Trap (NTR) – She is a best friend and just playing around, or is she? Two ladies who already have boyfriends discover they’re gay for each other.
From the New World (Shin Sekai Yori) – This takes place in a world where the culture is that everyone is bisexual and same-sex relationships are considered a normal and healthy part of growing up. So all the characters are cannon bisexuals and there are several same-sex relationships.
Aoi Hana (Sweet Blue Flowers) – a coming of age and growing up story about several lesbians in high school.
LOVELESS – a story about abuse and what it means to become an adult. the main character is in a gay relationship and there are several other gay and lesbian relationships included, most of the non-healthy variety.
Uragiri wa boku o namae o shiteiru (Uraboku) – a dark and angsty fighting show with gay men and their relationships also going on.
Togainu no Chi – Also a dark and angsty fighting show with gay men.
Super Lovers – A man ends up with an adopted brother he’s never met (that he knows) who comes to live with him as a teenager. Gay things ensue.
Princess Princess – a comedy about boys in an all-boys-school who dress up as “princesses” and are then lusted after by the general school body.
07 Ghost – There are many gay things and relationships here. It’s a alternate world fantasy show that’s mostly angsty and plot. The gay is a secondary.
Maria-sama ga Miteiru – A show about girls in an all-girls school who have relationships with each other.
Kyou Kara Maou! – the main character quickly and accidentally gets engaged to another man, and then stays that way for the rest of the series. There is some gay romance that ensues, though not necessarily canon pairings.
Monochrome Factor – Guy ends up in a fantastic fighting scene and develops a romance with another guy.
Yurikuma Arashi – a plethora of lesbian characters, both human and anthropomorphized bear.
No. 6 – a sci-fi series where the main character also has a gay relationship with another guy.
Revolutionary Girl Utena – the main female leads are in a lesbian-ish relationship (it’s canon in the movie and extras) as well as having a lesbian duelist (Juri) and lots and lots of homoeroticly suggestive male relationships. Plus, it fights the patriarchy!
Maria-Holic – the main character is a lesbian who goes to an all girls school so she can enjoy a gay gay school life, who has to deal with a jerk who is a crossdresser and maybe trans prolly not though. Played for laughs and often mostly for a het male audience, it’s still got a lot of gay going on.
Saki – These Mahjong girls are so gay. Sometimes it is mostly subtext, but also some outright are gay. Is more for a straight audience. The main part of the show is mahjong fights but there is a burgeoning lesbian romance.
Mouretsu Pirates – The plot mostly revolves around the discordance of cute girls running a pirate ship and being girls and how confining gender stereotypes can be. In general is a story about female empowerment and growing up. The importance of deep female relationships is stressed and gay hint hint gets bigger and gayer as the show goes on. Then there is a whole huge arc towards the end where they go to rescue a lesbian friend from being forced to marry a dude because of gender roles and shit, and in then end the lesbians share an adorable kiss of triumph and everyone cheers.
Strawberry Panic – Girls at an all girls school fall in love and have relationships.
These shows include gender bending, non-joke crossdressing, non-binary, or trans characters (this category is intentionally broad because trans representation in anime is murky and complicated)
Wandering Son (Hourou Musuko) – One of the best trans representations I’ve ever seen in anime. It’s a soft story about several children who are entering puberty and questioning what it means to do that. What it means to develop secondary sex characteristics and whether or not they want that. AFAB and AMAB characters go through this together.
Simoun – the story is about What would the world be like if people were all born female and then some became male after puberty, by choice or luck. And war and magic fighting, but there are a lot of lesbian kisses and stories that revolve around GENDER FEELS and gender development. Delves somewhat into the trans experience of having a gender assigned to you that you didn’t chose and how traumatic that can be.
Princess Jellyfish – the plot of this story revolves around the main female lead interacting with a genderqueer character, who is 100% a crossdresser and who can also be interpreted as trans or NB, depending on translations and interpretation.
Sailor Moon – Obviously it’s gender queering it up with Haruka and the Sailor Stars.
Paradise Kiss – Isabella is a trans woman, treated with dignity and respect and ends up happy.
Knights of Sidonia – Non-binary character in the main cast, has ability to become trans.
Rose of Versailles – Oscar, the main character is genderqueer, definitely a crossdresser and arguably a transman, but it is muddled.
Genshiken – there is a trans woman in the second season.
Kino’s Journey – Titular character is non binary.
Tokyo Godfathers – There is a older trans woman in the main cast who gets treated decently.
These shows have LGBT representation in the primary cast.
Jormungand – Valmet is a lesbian in love with the main female lead, Koko. It’s a side storyline, but it’s still a major part of the main cast and some story arcs.
Sailor Moon – Uranus and Neptune! Also Zoisite and Kunzite!
To Aru Kagaku no Railgun – It’s got a lesbian supporting character and the development of her one-sided relationship is important to the show.
Miss Kobayashi’s Dragon Maid – It is left unclear whether or not Kobayashi is in a platonic or romantic relationship with Tohru, but Tohru is definitely a lesbian and attracted to and pursuing Kobayashi. An adorable and heartwarming show!
Psycho-Pass – two of the main characters in the second season are in a lesbian relationship. An intense and dark sci-fi show!
Haruchika – One of the two leads is a gay boy.
Fuuka – One of the male band members is openly gay
Yami no Matsuei – The male villain is attracted to other men and there are homoerotic things going on everywhere
The Melancholy of Suzumiya Haruhi – Haruhi is cannon Bisexual.
Claymore – There are a lot of lesbian subtexts here. The story in general is about the clashing of feminine stereotypes and yet monstrous nature of the fighter women. Gender roles! Women loving each other!
Kuroshitsuji (Black Butler) – Whether Sebastian wants to eat or eat Ciel is left ambiguous but Grell and other repeating characters are explicitly gay.
Tanaka-kun is Always Listless – A character confesses love to another lady character early in the show.
These shows have at least plausible deniability (so they’re not quite rep, not quite not LGBT either though) but is LGBT in the subtext, because of press releases or other supplements, etc. This category includes thing that can be considered queerbait or gay-in-the-fanservice-only.
Madlax, Noin, and El Cazador De La Bruja – The main female leads are in a strong lesbian interpretable but not necessarily outright gay relationship. The story hinges on lady bonding and having a deep connection. Lots of fanservice that is suggestive. This is three different shows but they are all SO SIMILAR that I am clumping them rather than write this three times.
Neon Genesis Evangelion – Shinji is bisexual in the extended universe and some depictions and only assumed heterosexual in the others. One can assume he’s always bisexual and it just doesn’t come up much explicitly.
Attack on Titan – Ymir and Christa are confirmed in a lesbian relationship but only outside the show content itself.
D.N.Angel – Nothing overtly gay, but fanservice signals gay relationships between the main male characters.
Cardcaptor Sakura – The main character’s brother and his best friend appear to be in a gay relationship off screen, but it isn’t explicitly in the show.
Free! Iwatobi Swim Club – There is nothing overtly gay per se, but there is queerbaiting and female-gaze centric fanservice galore.
Sound Euphonium – Another show that queer baits. Nothing is ever explicit but there is a lot of lesbian relationship fanservice/hinting.
Ouran High School Host Club – The characters do a lot of fanservice that is gay suggestive, both for the audience and for other characters in the show.
Puella Magi Madoka Magica – A lot of the relationships between women here can be taken gayer or not at all gay, and there are fanservice things that are gay.
Kids on the Slope (Sakamichi no Apollon) – The main character is seemingly in love with another boy (and therefore hinted bisexual) and there are queerbaiting moments but nothing happens or is confirmed in canon.
Amanchu! – The main character slowly becomes more enamored with another girl and they have soft and loving moments. It can easily be read as a slow burn lesbian love story, though there is plausible deniability about whether the love they share is platonic or romantic.
Supporting or Minor character or plot is cannon LGBT
Hunter x Hunter – Trans girl in the supporting cast.
Tiger and Bunny – genderqueer and gay secondary character.
Gankutsuou: The Count of Monte Cristo – Lots of male homoerotic overtones of the Count, plus a secondary trans girl character
Michiko and Hatchin – several genderqueer and gay side characters
LGBT shows I haven’t seen or can’t remember and so can’t classify in above categories
Yuru Yuri – there are lesbians?
Showa Genroku Rakugo Shinju – gay male queerbait?
Kikuhiko – ?
Samurai Flamenco – gay guy?
Mikagura School Suite – has a lesbian?
Monogatari series – Kanbaru?
Gatchaman Crowds – has a tran character?
Love Live – ?
Symphogear – Queerbaiting?
This Boy Caught a Merman
This Boy is a Professional Wizard – I know it’s gay but that’s it.
Riddle Story of Devil